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To investigate genetic features and the allele distribution of transferrin gene in three Cheju horse groups(group Ⅰ, 137 horses of Jeju institute; group Ⅱ, 107 horses of farms; group Ⅲ, 89 racing horses) and three foreign breeds(103 Thoroughbred, 10 Mongolian and 5 American Quarter horses), transferrin gene exons 13, 15, and 16 were analyzed by SSCP. The allele frequencies of transferrin gene of these groups and breeds were used to calculate genetic distances and to test population differentiations. The Fst values were 0.067 between Cheju horse groups Ⅰ and Ⅱ, 0.070 between Cheju horse groups Ⅰ and group Ⅲ, 0.091 between Cheju horse group Ⅰ and Mongolian breed, and 0.189 between Cheju horse group Ⅰ and Thoroughbred breed, Cheju horse group Ⅰ showed significant population differentiation from other two Cheju horse groups and three foreign breeds while Cheju horse group Ⅲ showed significant population differentiation only from Cheju horse group Ⅰ and Thoroughbred breed)p,0.05). Results indicate that three Cheju horse groups showed population differentiation between each other, suggesting genetic heterogeneity of Cheju horses.
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